Thermo Fisher Scientific EBI3 Polyclonal Antibody

Applications

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot contents and freeze at -20°C or below.
• Avoid freeze/thaw cycles.
• Centrifuge product if not completely clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

This antibody is specific for mouse and rat EBI-3 protein.
No cross-reactivity with human EBI-3 has been observed.
Cross-reactivity with IL-27 from other sources has not been determined.

Target Information

EBI3 (Epstein-Barr virus Induced-3) is a secreted glycoprotein of the hematopoietin receptor family.
It plays a regulatory role in Th2-type immune responses and Th2-mediated tissue inflammation, possibly via iNKT cell function control.
EBI3 dimerizes with p28 and p35 subunits of IL-12 to form IL-27 and IL-35, respectively.
IL-27, produced by activated antigen-presenting cells upon TLR ligation, negatively regulates Th17 cell differentiation.
EBI3 expression in dendritic cells is transcriptionally regulated by TLR signaling via MyD88 and NF-κB.
EBI3 signaling suppresses IL-17 production and induces IL-10 hyperproduction, inhibiting delayed-type hypersensitivity responses.
It may also play a role in controlling tumor metastasis via lung CD8+ T cells.
EBI3 deficiency is linked to reduced Th2 cytokine production and decreased allergic airway inflammation in asthma.
Targeted deletion of EBI3 protects mice from lung metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.