Thermo Fisher Scientific IL-18 Polyclonal Antibody
마우스 IL-18 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ELISA, IP, Neutralization 등 다양한 응용에 사용 가능. 단백질 A로 정제된 액상 형태이며, 보존제 무첨가. -20°C에서 보관하며 반복 동결·해동을 피해야 함.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC-F) | 1:50–1:200 |
| ELISA | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 1:500–1:2,000 |
| Neutralization (Neu) | 1:50–1:400 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Native 157 aa mouse IL-18 produced in E. coli; whole rabbit serum used for immunization |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot and freeze at -20°C or below for long-term storage.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only immediately before use.
This antibody primarily recognizes mature 18 kDa mouse IL-18 and is useful in detecting its presence in various assays.
It also recognizes the 24 kDa inactive precursor form of mouse IL-18 and can detect rat IL-18 using similar dilutions.
A control of similarly diluted LOW ENDOTOXIN CONTROL RABBIT IgG (code #011-001-297) is recommended.
Target Information
IL-18 is a proinflammatory cytokine that induces IFN-γ production in T cells.
In combination with IL-12, it inhibits IL-4-dependent IgE and IgG1 production and enhances IgG2a production in B cells.
IL-18 binding protein interacts specifically with IL-18, regulating its biological activity negatively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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