Thermo Fisher Scientific IL-27/p28 Polyclonal Antibody, HRP
Thermo Fisher Scientific의 IL-27/p28 Polyclonal Antibody, HRP는 Mouse IL-27/p28 단백질에 특이적인 항체로 WB, IHC, ELISA에 적합합니다. HRP 결합형이며 고순도의 rabbit IgG로 제조되었습니다. 안정적인 장기 보관 및 높은 특이성을 제공합니다.
- 카탈로그번호
- 210-403-B54
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,500 |
| ELISA | 1:10,000–1:50,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length recombinant mouse IL27/p28 protein. |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature.
Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is specific for mouse IL-27/p28 protein. A BLAST analysis suggests cross-reactivity with IL-27A/p28 from mouse sources based on 100% homology with the immunizing sequence. Based on 90% or greater positive homology, cross-reactivity to rat is possible. Cross-reactivity with IL-27 from other sources has not been determined.
Target Information
IL-27, a member of the IL-12 family, is a heterodimeric protein composed of the p40-related protein EBI3 and the IL-12p35-related protein p28 (also known as IL-30).
Produced by activated APCs and mature dendritic cells, IL-27 acts on NK cells and naive CD4+ T cells.
Binding of IL-27 to WSX-1/gp130 activates JAK1, STAT1, and STAT3 phosphorylation.
WSX-1/TCCR-deficient mice show impaired Th1 responses and increased susceptibility to L. monocytogenes, indicating IL-27’s role in Th1 initiation.
IL-27 induces Th1-specific transcription factor T-bet and suppresses Th2-specific GATA-3.
It also exhibits potent antitumor activity by enhancing CTL generation and inhibiting tumor growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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