Thermo Fisher Scientific IL-27/p28 Polyclonal Antibody
마우스 IL-27/p28 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체입니다. WB 및 ELISA에 적합하며, 고순도 이온교환 크로마토그래피로 정제되었습니다. 비결합형 형태로 동결건조 상태로 제공되며, 장기 보관 시 -20°C에서 안정적입니다.
- 카탈로그번호
- 210-401-B54
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-27/p28 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1:1,000–1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length recombinant mouse IL-27/p28 protein. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Product is stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This antibody is specific for mouse IL-27/p28 protein.
A BLAST analysis suggests cross-reactivity with IL-27A/p28 from mouse sources (100% homology with the immunizing sequence).
Based on ≥90% homology, possible cross-reactivity with rat IL-27.
Cross-reactivity with other species has not been determined.
Target Information
IL-27, a member of the IL-12 family, is a heterodimeric protein consisting of the p40-related protein Epstein-Barr virus-induced gene 3 (EBI3) non-covalently linked to an IL-12p35-related protein, p28 (also known as IL-30).
IL-27 is produced by activated APCs and mature dendritic cells, acting on NK cells and naïve CD4+ T cells.
Binding of IL-27 to WSX-1/gp130 activates JAK1, STAT1, and STAT3, leading to STAT1/3 phosphorylation.
WSX-1/TCCR-deficient mice show impaired Th1 responses and increased susceptibility to L. monocytogenes infection, indicating IL-27’s role in initiating Th1 responses.
IL-27 induces the Th1-specific transcription factor T-bet and suppresses GATA-3, contributing to Th1 differentiation.
Recent studies show IL-27 exhibits antitumor activity by enhancing CTL activity, inhibiting tumor growth, and inducing regression of neuroblastoma tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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