Thermo Fisher Scientific LGI1 Monoclonal Antibody (N283/7)
LGI1 단백질을 검출하기 위한 Mouse monoclonal 항체로, Western blot, IHC, ICC/IF에 적합. Human, Mouse, Rat 시료에 반응하며 비결합(Unconjugated) 형태로 제공. Protein G 정제, PBS/glycerol buffer 보존, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:100 | |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N283/7 |
| Immunogen | Fusion protein amino acids 37–113 (LRRNT domain and first LRR repeat) of mouse LGI1. Rat: 100% identity (77/77 amino acids identical). Human: 98% identity (76/77 amino acids identical). ~50% identity with LGI2, LGI3, and LGI4. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735259 |
Additional Formats
Product Specific Information
- 1 µg/mL of MA5-27652 was sufficient for detection of LGI1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- Detects approximately 60 kDa.
- This antibody was formerly sold as clone S283-7.
Target Information
The leucine-rich, glioma inactivated gene 1 (LGI1) was first identified as a candidate tumor suppressor gene for glioma and may play a role in other cancers. LGI1 is a member of a family of highly related proteins containing leucine-rich repeats (LRRs), which are similar to transmembrane signaling molecules and receptors. LGI1 serves as a ligand to ADAM22, a metalloprotease localized at the synapse. Mutations in LGI1 account for nearly half of autosomal dominant lateral temporal epilepsy (ADTLE), characterized by focal seizures with predominant auditory symptoms. Two isoforms of LGI1 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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