Thermo Fisher Scientific LGI1 Monoclonal Antibody (N283/7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:100

-

Immunocytochemistry (ICC/IF)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

N283/7

Immunogen

Fusion protein amino acids 37-113 (LRRNT domain and first LRR repeat) of mouse LGI1. Rat: 100% identity (77/77 amino acids identical). Human: 98% identity (76/77 amino acids identical). ~50% identity with LGI2, LGI3 and LGI4. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:LGI1,uniProtId:Q9JIA1-1,ncbiNodeId:10090,antigenRange:37-113,antigenLength:557,antigenImageFileName:MA5-27652_LGI1_Q9JIA1-1_House_mouse.svg,antigenImageFileNamePDP:MA5-27652_LGI1_Q9JIA1-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• APC
• FITC
• PE
• PerCP
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2735259

Product Specific Information

1 µg/mL of MA5-27652 was sufficient for detection of LGI1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 60kDa.

This antibody was formerly sold as clone S283-7.

Target Information

The leucine-rich, glioma inactivated gene 1 (LGI1) was first identified as a candidate tumor suppressor gene for glioma and may play a role in other cancers. LGI1 is a member of a family of highly related proteins containing leucine-rich repeats (LRRs) which are highly similar to other transmembrane signaling molecules and receptors. LGI1 serves as a ligand to ADAM22, a metalloprotease localized at the synapse. Mutations in LGI1 account for nearly half of autodominant lateral temporal epilepsy (ADTLE), an epileptic syndrome characterized by focal seizures with predominant auditory symptoms. Two isoforms of LGI1 are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.