Thermo Fisher Scientific Pan SHANK Monoclonal Antibody (N23b/49)
Pan SHANK 단백질을 인식하는 Mouse monoclonal antibody로, Human, Mouse, Rat에 반응합니다. Western blot, IHC, ICC, IP, AM 등 다양한 응용에 사용 가능하며, 단백질 농도는 1 mg/mL입니다. Protein G로 정제되었으며 -20°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 | |
| Immunocytochemistry (ICC/IF) | 1:100 | |
| Immunoprecipitation (IP) | Assay-dependent | |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N23b/49 |
| Immunogen | Fusion protein corresponding to amino acids 84–309 of rat Shank2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735302 |
Available Conjugated Formats
Product Specific Information
1 µg/mL of MA5-27633 was sufficient for detection of Shank1–4 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 160 kDa. Recognizes Shank1, Shank2, and Shank3.
Target Information
Shank proteins are a family of scaffold proteins involved in interactions with various membrane and cytoplasmic proteins. They are localized at postsynaptic sites of excitatory synapses and play key roles in signal transmission within postsynaptic neurons.
Studies suggest that Shank2 is expressed in developing retinal neurons and may contribute to neuronal differentiation. Disruption of glutamate receptors at the Shank postsynaptic platform has been implicated in postsynaptic density degradation, leading to synaptic dysfunction and loss in Alzheimer’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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