Thermo Fisher Scientific Myc Tag Monoclonal Antibody, AP

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:5,000
• Publications: View 2 publications

Product Specifications

Product Specific Information

• NOTE: R952-25 was previously sold under product #46-0285. When re-ordering, please use #R952-25.
• Recognizes amino acid sequence: Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-.
• Contains enough material for 25 Western blots.
• In Western blot with 100 ng recombinant Positope™ control protein, a detectable signal was observed within 15 minutes using a chromogenic substrate.
• The Positope™ control protein (53 kDa) contains seven epitope tags, including His(C-term), HisG, c-Myc, and V5.
• For Western blot, dilute AP-conjugated antibody in Tris-Buffered Saline (TBS) containing 0.1% Tween-20 and 1% nonfat dry milk.
• No cross-reactivity observed in bacterial lysates.

Target Information

Myc is a proto-oncogene encoding a nuclear phosphoprotein involved in cell cycle progression, apoptosis, and cellular transformation.
It forms a heterodimer with transcription factor MAX to bind E-box DNA sequences and regulate gene transcription.
Gene amplification is frequently observed in human cancers, and translocations are linked to Burkitt lymphoma and multiple myeloma.
Translation may initiate from both a non-AUG (CUG) and AUG site, producing two isoforms with distinct N-termini.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.