Thermo Fisher Scientific Myc Tag Monoclonal Antibody, FITC

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:500

View 6 publications 6 publications

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Immunogen

c-Myc synthetic peptide: Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.01% sodium azide

Storage conditions

4° C, store in dark

RRID

AB_2556563

Product Specific Information

NOTE: R953-25 was previously sold under product #46-0307. When re-ordering, please use #R953-25.

R953-25 recognizes amino acid sequence: Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-.

This antibody has been tested in IF experiments using cultured CHO (Chinese Hamster ovary) cells expressing recombinant epitope-tagged fusion proteins. For IF, dilute the FITC-conjugated antibody in PBS containing 10% fetal bovine serum (FBS). The amount of antibody provided is sufficient for 25 immunostaining reactions using a 1 mL working solution per reaction.

This antibody has also been tested against purified Positope™ control protein. The Positope™ control protein is a 53 kDa recombinant protein that contains seven epitope tags, including His(C-term), HisG, c-myc, and V5.

Target Information

Myc is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.