Thermo Fisher Scientific Myc Tag Monoclonal Antibody, FITC
FITC 형광 표지된 c-Myc 태그 단클론 항체로, WB, ICC/IF, IP 등 다양한 응용에 사용 가능. CHO 세포 및 Positope™ 단백질 테스트 완료. 1 mg/mL 농도의 액상 형태이며, 4°C 암소 보관. 연구용으로만 사용.
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Thermo Fisher Scientific Myc Tag Monoclonal Antibody, FITC
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 | View 3 publications |
| Immunocytochemistry (ICC/IF) | 1:500 | View 6 publications |
| Immunoprecipitation (IP) | – | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | c-Myc synthetic peptide: Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu- |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS |
| Contains | 0.01% sodium azide |
| Storage Conditions | 4°C, store in dark |
| RRID | AB_2556563 |
Product Specific Information
- Catalog #: R953-25 (previously sold as #46-0307)
- Recognizes amino acid sequence: Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-
- Tested in IF using CHO cells expressing recombinant epitope-tagged fusion proteins.
- For IF, dilute in PBS containing 10% fetal bovine serum (FBS).
- Sufficient for 25 immunostaining reactions (1 mL working solution per reaction).
- Tested against purified Positope™ control protein (53 kDa recombinant protein containing seven epitope tags, including His(C-term), HisG, c-Myc, and V5).
Target Information
Myc is a proto-oncogene encoding a nuclear phosphoprotein involved in cell cycle progression, apoptosis, and cellular transformation. It forms a heterodimer with MAX, binding to the E-box DNA consensus sequence to regulate transcription of target genes.
Amplification of Myc is common in human cancers, and translocations are associated with Burkitt lymphoma and multiple myeloma. Translation initiates from both an upstream non-AUG (CUG) and a downstream AUG start site, producing two isoforms with distinct N-termini.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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