Thermo Fisher Scientific Myc Tag Monoclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 49 publications 49 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 15 publications 15 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 3 publications 3 publications

Immunoprecipitation (IP)

-

View 11 publications 11 publications

ChIP assay (ChIP)

-

View 2 publications 2 publications

Gel Shift (GS)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Immunogen

c-Myc synthetic peptide: Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

~1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2556560

Product Specific Information

NOTE: R950-25 was previously sold under product #46-0603. When re-ordering, please use #R950-25.

This antibody recognizes amino acid sequence: Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-.

This product contains enough material for 25 Western blots. Western blot against 500 ng of recombinant protein gave a strong signal using chemiluminescent or alkaline phosphatase detection reagents. For Western blot, dilute in PBS containing 0.05% Tween-20 and 5% nonfat, dry milk (PBSTM).

Target Information

Myc is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.