Thermo Fisher Scientific c-Cbl Monoclonal Antibody (3B12)
Human, Mouse, Rat에 반응하는 c-Cbl 단일클론 항체로, WB, IHC, ICC, Flow, ELISA에 사용 가능. Unconjugated 형식으로 제공되며, 4°C 단기 보관 및 -20°C 장기 보관 권장. 약 120kDa의 C-CBL 단백질 검출에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 | - |
| Flow Cytometry (Flow) | 1:200–1:400 | 1 publication |
| ELISA | 1:10,000 | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3B12 |
| Immunogen | Purified recombinant fragment of human C-CBL expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Storage Buffer | Ascites |
| Contains | 0.03% Sodium Azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet Ice |
| RRID | AB_11153576 |
Product Specific Information
MA5-15885 targets C-CBL in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human, Mouse, and Rat samples.
The immunogen is a purified recombinant fragment of human C-CBL expressed in E. coli.
MA5-15885 detects C-CBL, which has a predicted molecular weight of approximately 120 kDa.
Target Information
Cbl proteins are a family of ubiquitin protein ligases (E3s) that negatively regulate signaling by targeting activated tyrosine kinases for degradation.
Cbl-c is the most recently cloned member of the Cbl proteins and is expressed only in epithelial cells, whereas other Cbl proteins are ubiquitously expressed.
Cbl-c, like the other mammalian Cbl proteins, can ubiquitinate the activated EGFR and target it for degradation. Through interactions with proteins containing SRC homology-2 (SH2) and SH3 domains, CBL proteins modulate downstream cell signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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