Thermo Fisher Scientific c-Jun Monoclonal Antibody (5B1)
c-Jun 단백질을 검출하기 위한 Mouse monoclonal antibody (Clone 5B1). Western blot, IHC, ICC/IF, Flow cytometry, ELISA, ChIP 등 다양한 응용에 적합. Human, Mouse, Non-human primate 시료에 반응. 43kDa c-Jun 단백질을 인식하며 연구용으로 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 5 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 | - |
| Flow Cytometry (Flow) | 1:200–1:400 | - |
| ELISA | 1:10,000 | - |
| ChIP assay (ChIP) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5B1 |
| Immunogen | Purified recombinant fragment of human c-Jun expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C for short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11153582 |
Product Specific Information
MA5-15881 targets c-Jun in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human, Mouse, and Non-human primate samples.
The immunogen is a purified recombinant fragment of human c-Jun expressed in E. coli.
MA5-15881 detects c-Jun, which has a predicted molecular weight of approximately 43 kDa.
Target Information
c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.
It promotes the activity of NR5A1 when phosphorylated by HIPK3, leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
It is also involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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