Thermo Fisher Scientific c-Jun Monoclonal Antibody (5B1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

-

Immunocytochemistry (ICC/IF)

1:200-1:1,000

-

Flow Cytometry (Flow)

1:200-1:400

-

ELISA (ELISA)

1:10,000

-

ChIP assay (ChIP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5B1

Immunogen

Purified recombinant fragment of human c-Jun expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. not determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_11153582

Product Specific Information

MA5-15881 targets c-Jun in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human, mouse, and Non-human primate samples.

The MA5-15881 immunogen is purified recombinant fragment of human c-Jun expressed in E. Coli. .

MA5-15881 detects c-Jun which has a predicted molecular weight of approximately 43kDa.

Target Information

c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5-TGA\[CG\]TCA-3. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.