Thermo Fisher Scientific PSMB8 Polyclonal Antibody
Rabbit polyclonal antibody targeting human PSMB8 (Proteasome 20S LMP7). Validated for WB and ICC/IF applications. Antigen affinity purified, supplied in PBS with BSA. Suitable for immunoproteasome research. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–3 µg/mL | View 4 publications |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic Peptide: C V(259) E S T D V S D L L H Q Y R E A(274) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2172350 |
Product Specific Information
PA1-972 detects Proteasome 20S LMP7 in western blot and immunofluorescence.
PA1-972 can be used with blocking peptide PEP-132.
Target Information
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure composed of four rings of 28 non-identical subunits: two rings of seven alpha subunits and two rings of seven beta subunits. Proteasomes are abundant throughout eukaryotic cells and cleave peptides in an ATP/ubiquitin-dependent, non-lysosomal pathway.
The immunoproteasome, a modified form, processes class I MHC peptides. The PSMB8 gene encodes a proteasome B-type (T1B family) 20S core beta subunit located in the MHC class II region. Expression is induced by gamma interferon, replacing the catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing generates the mature subunit. Two transcript variants encode isoforms that yield the same mature protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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