Thermo Fisher Scientific PSMD3 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

PA1-974 detects proteasome 19S subunit S3 from human and mouse cells.
It has been successfully used in ICC/IF, FACS, and Western blot procedures.
By Western blot, this antibody detects an approximately 60 kDa protein representing proteasome 19S subunit S3 from HeLa cell extract.
For Western blot procedures, it is necessary to transfer the protein to membrane at pH 11.
The immunizing peptide corresponds to amino acid residues 513–534 from human proteasome 19S subunit S3 protein.

Target Information

The 26S proteasome is a multicatalytic proteinase complex composed of two subcomplexes: a 20S core and a 19S regulator.
The 20S core consists of four rings of 28 non-identical subunits (two rings of seven alpha and two rings of seven beta subunits).
The 19S regulator includes a base with six ATPase and two non-ATPase subunits, and a lid with up to ten non-ATPase subunits.
Proteasomes are abundant in eukaryotic cells and cleave peptides in an ATP/ubiquitin-dependent, non-lysosomal process.
The immunoproteasome plays a key role in processing class I MHC peptides.
This gene encodes one of the non-ATPase subunits of the 19S regulator lid.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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