Thermo Fisher Scientific IL-2 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFAQSII STLT

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human IL-2. Anti-Human IL-2-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hIL-2 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-2 (500-P22G) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hIL-2.

Western Blot:
To detect hIL-2 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hIL-2 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P22GBT-1MG will be provided as 2 × 500 µg.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes, and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, representing an unusual regulatory mode for precise gene expression control. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease, suggesting an essential role of this gene in immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.