Thermo Fisher Scientific IL-2 Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL
• Publications: 1 publication available

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFAQSII STLT.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-2. Anti-Human IL-2-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hIL-2 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-2 (500-P22) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hIL-2.

Western Blot:
To detect hIL-2 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
Used with compatible secondary reagents, the detection limit for Recombinant hIL-2 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P22BT-1MG will be provided as 2 × 500 µg.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes, and macrophages.
IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by IL-4 and IL-7.
Expression of the IL-2 gene in mature thymocytes is monoallelic, representing an unusual regulatory mechanism for precise gene expression.
Disruption of an IL-2-like gene in mice leads to an ulcerative colitis-like disease, suggesting an essential role of IL-2 in immune response to antigenic stimuli.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.