Thermo Fisher Scientific Coagulation Factor VIII (Cleaved form, C-Term) Polyclonal Antibody
사람 Coagulation Factor VIII(C-Term, Cleaved form)을 인식하는 Rabbit Polyclonal 항체. Western blot과 IHC(P)에서 사용 가능. 항원 친화 크로마토그래피로 정제되었으며 PBS buffer에 0.01% sodium azide 포함. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to the C-terminal region of the cleaved form around sequence EITRTTLQS of human FVIII protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Human colon carcinoma
- Cellular location: Secreted, extracellular space
Target Information
This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation. Factor VIII acts as a cofactor for factor IXa, which, in the presence of Ca²⁺ and phospholipids, converts factor X to the activated form Xa.
This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein (isoform a) that circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a smaller protein (isoform b) that primarily consists of the phospholipid binding domain of factor VIIIc, essential for coagulant activity.
Defects in this gene result in hemophilia A, a common recessive X-linked coagulation disorder.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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