Thermo Fisher Scientific PIGR Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human pIgR is observed.
• Reconstitute at 0.2 mg/mL in sterile PBS.
• Endotoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

Polymeric IgA and IgM are produced and secreted by B cells in the lamina propria beneath the mucosal lining of polarized epithelial cells. Polymeric immunoglobulin receptors (pIgRs) are expressed on the basolateral surface of glandular epithelia and mediate the transcellular transport of secretory immunoglobulin polymers across the epithelium.

During this process, pIgR undergoes proteolytic cleavage to generate a fragment called the secretory component (SC). When immunoglobulin polymers associate with SC, they become resistant to enzymatic degradation during transcytosis. SC and pIgR are crucial for proper mucosal immunity, acting as molecular chaperones that maintain polymeric Igs intact as they enter body fluids.

The human SC (pIgR) gene maps to chromosome 1q31–q41 and encodes a 764 amino acid protein. The receptor contains five units homologous to immunoglobulin variable (V) regions and a transmembrane region sharing homology with certain immunoglobulin variable regions.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.