Thermo Fisher Scientific Human IFN-gamma Recombinant Protein

Applications

• Control (Ctrl): Assay-dependent

Product Specifications

Product Specific Information

Recombinant human IFN-gamma is a non-glycosylated protein containing 144 amino acids.
Reconstitute using sterile water at 0.1 mg/mL and centrifuge prior to opening vial. Gently pipet solution down the sides of the vial. Do not vortex the sample.
Store reconstituted material at -20°C and add 0.1% BSA for additional stability.

Target Information

IFN-gamma (Interferon gamma, Type II interferon) is a macrophage activation factor and immune interferon produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemagglutinin, and other cytokines.
It is a dimeric protein consisting of two 146 amino acid subunits and functions as a homodimer (~45 kDa). On SDS-PAGE, IFN-gamma appears as 25, 20, and minor 15.5 kDa bands due to differential glycosylation.
Human IFN-gamma is species-specific and does not cross-react with mouse IFN-gamma.

Functions:

• Antiviral and tumor antiproliferative activity
• Induction of class I and II MHC
• Macrophage activation
• Enhancement of immunoglobulin secretion by B lymphocytes
• Cytokine regulation and synergistic action with other cytokines

Activation occurs through binding to IFN-gamma receptor I and II, activating the JAK-STAT pathway.
Human IFN-gamma shares about 40% sequence homology with mouse IFN-gamma.
Expression is upregulated by IL-2, FGF basic, EGF, and downregulated by vitamin D3 or DMN.
Mutations in the IFN-gamma gene are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.