Thermo Fisher Scientific CD150 Monoclonal Antibody (9D1), PE-Cyanine7, eBioscience
Mouse CD150(SLAM) 단백질을 인식하는 9D1 클론의 Rat IgG1 단일클론 항체로, PE-Cyanine7 형광체로 결합된 Flow Cytometry용 시약입니다. 면역세포 활성화 및 HSC 분석에 적합하며, 광민감성이 있으므로 암조건에서 보관해야 합니다.
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Thermo Fisher Scientific CD150 Monoclonal Antibody (9D1), PE-Cyanine7, eBioscience
Applications and Tested Dilution
- Flow Cytometry (Flow): 1.0 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9D1 |
| Conjugate | PE-Cyanine7 |
| Excitation/Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2802248 |
Additional Formats
Product Specific Information
The 9D1 monoclonal antibody reacts with mouse CD150, a ~70 kDa transmembrane glycoprotein known as Signaling Lymphocyte Activation Molecule (SLAM). CD150 is expressed by T (especially TH1) and B cells, and its expression is rapidly upregulated upon activation. Immature thymocytes and dendritic cells also express this antigen. Signaling through SLAM induces proliferation and enhances interferon-gamma response, and plays a role in T cell–APC adhesion. 9D1 is reported to be an activating antibody.
Mouse hematopoietic stem cells (HSC) can be identified using SLAM family markers (CD150⁺CD244⁻CD48⁻). For this application, antibody clone mShad150 (Product #12-1502) is recommended.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes; ≤1.0 µg/test (100 µL final volume). Optimize titration for best performance.
Light sensitivity: Tandem dye is photo-sensitive; protect vial and samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) for up to 3 days at 4°C in the dark with minimal brightness impact.
Excitation: 488–561 nm
Emission: 775 nm
Compatible Lasers: Blue, Green, Yellow-Green
Target Information
CD150 (SLAM, Signaling Lymphocyte Activation Molecule) is part of the immunoglobulin superfamily and involved in T cell stimulation. It is constitutively expressed on peripheral memory T cells, immature thymocytes, and some B cells, and rapidly induced on naive T cells after activation. SLAM signaling enhances B cell expansion and differentiation. The extracellular domain serves as a receptor for measles virus and interacts with SH2D1A and PTPN11. Mutations in CD150 may be linked to X-linked lymphoproliferative disease (XLP).
For Research Use Only. Not for diagnostic use or resale without authorization.
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