Thermo Fisher Scientific Histone H2B Recombinant Rabbit Monoclonal Antibody (RM230)
Histone H2B 단백질을 인식하는 재조합 토끼 단클론 항체로, 높은 특이성과 재현성을 제공합니다. Western blot, ICC/IF, ELISA, Luminex 등 다양한 응용에 적합하며, 로트 간 일관성과 동물 유래 성분이 없는 포뮬레이션을 특징으로 합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–1 µg/mL |
| ELISA | 0.2–1 µg/mL |
| Luminex (LUM) | 0.2–1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM230 |
| Immunogen | Peptide corresponding to the C-terminus of human Histone H2B |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS (pH 7.2–7.4), with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2661894 |
Product Specific Information
This antibody reacts to the Histone H2B protein, independent of post-translational modifications, and shows no cross-reactivity with other histone proteins.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to rabbit immune repertoire
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fibers in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of the four core histones (H2A, H2B, H3, and H4). These structures further compact DNA into chromatin, regulating accessibility for transcription, repair, replication, and maintaining chromosomal stability.
Histone H2B also exhibits broad antibacterial activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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