Thermo Fisher Scientific H2BK20ac Recombinant Rabbit Monoclonal Antibody (RM235)
Histone H2B Lys20 acetyl화 특이적 항체로, WB, ICC, ELISA, ChIP, Luminex 등 다양한 응용에 적합. 재조합 토끼 단클론 항체로 높은 특이성과 재현성 제공. HEK293 발현 시스템 기반, 1 mg/mL 농도의 액상 형태. -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| ELISA | 0.2–1 µg/mL |
| ChIP assay (ChIP) | 1.5 µg / 1×10⁶ cells |
| Luminex (LUM) | 0.1–0.5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM235 |
| Immunogen | Acetyl-peptide corresponding to Acetyl-Histone H2B (Lys20) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS (pH 7.2–7.4) with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2661895 |
Product Specific Information
This antibody specifically recognizes Histone H2B acetylated at Lysine 20 (K20ac).
No cross-reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning antibody DNA sequences from immunoreactive rabbits.
Individual clones are screened to select optimal production candidates.
Advantages of recombinant rabbit monoclonal antibodies:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to large rabbit immune repertoire
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of eukaryotic chromatin.
Nucleosomes consist of ~146 bp of DNA wrapped around a histone octamer composed of pairs of H2A, H2B, H3, and H4.
These structures compact DNA into chromatin, regulating accessibility for transcription, replication, and repair.
Histone H2B also exhibits broad antibacterial activity.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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