Thermo Fisher Scientific KAP1 (TRIM28) Monoclonal Antibody (OTI7A4)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

The KRAB (Kruppel-associated box) domain (~45 amino acids) is a transcriptional repression domain found in many transcription factors. Over 220 KRAB-zinc finger protein (KRAB-ZFP) genes exist in the human genome. These proteins repress transcription through interactions with KAP-1 (KRAB-associated protein 1).
KAP-1 is an 835 amino acid polypeptide with a RING finger, B boxes, and a PHD finger. It forms complexes with KRAB-domain transcription factors to enhance repression efficiency. KAP-1 interacts directly with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1), targeting KRAZ1 to centromeric heterochromatin regions containing HP1alpha, thereby regulating transcriptional repression.
Mutations in KAP-1 that retain KRAB binding but lose HP1 interaction result in random KRAZ1 distribution and strong transcriptional activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.