Thermo Fisher Scientific KAP1 (TRIM28) Monoclonal Antibody (OTI8B12)

Thermo Fisher Scientific KAP1 (TRIM28) Monoclonal Antibody (OTI8B12)

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

The KRAB (Kruppel-associated box) domain, approximately 45 amino acids long, acts as a transcriptional repression domain found in many transcription factors. Over 220 KRAB-zinc finger protein (KRAB-ZFP) genes have been identified in the human genome.
These proteins repress transcription through interactions with KAP-1 (KRAB-associated protein 1), an 835 amino acid polypeptide containing a RING finger, B boxes, and a PHD finger.
KAP-1 forms complexes with KRAB-domain transcription factors to enhance repression and directly interacts with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1).
KAP-1 directs KRAZ1 to centromeric heterochromatin foci containing HP1alpha, regulating transcriptional repression.
Mutant KAP-1 proteins that bind KRAB but not HP1 result in random KRAZ1 distribution and strong transcriptional activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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