Thermo Fisher Scientific KAP1 (TRIM28) Monoclonal Antibody (OTI5E10)

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

The KRAB (Kruppel-associated box) domain (~45 amino acids) is a transcriptional repression domain found in many transcription factors.
Over 220 KRAB-zinc finger protein (KRAB-ZFP) genes exist in the human genome, functioning to repress transcription through interactions with KAP-1 (KRAB-associated protein 1).

KAP-1 is an 835 amino acid polypeptide containing a RING finger, B boxes, and a PHD finger. It forms complexes with KRAB-domain transcription factors to enhance repression efficiency.
KAP-1 directly interacts with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1), targeting KRAZ1 to centromeric heterochromatin regions containing HP1α.
Mutant KAP-1 that binds KRAB but not HP1 leads to random KRAZ1 distribution and strong transcriptional activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.