Thermo Fisher Scientific CD44v6 Monoclonal Antibody (VFF-7)
CD44v6 단백질을 인식하는 Mouse monoclonal antibody (Clone VFF-7). Western blot, IHC, Flow cytometry 등에 사용 가능. 인간 시료에 반응하며, 세포 표면 CD44 변이형 연구에 적합. 비결합형 액상 형태로 제공되며, 장기 보관 시 -20°C 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 | 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:400–1:500 | - |
| Flow Cytometry (Flow) | 1:50–1:200 | 1 publication |
| Blocking Assay (BLOCK) | - | 1 publication |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | VFF-7 |
| Immunogen | Fusion protein corresponding to the variable domains (v3 to v10) of human CD44 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS |
| Contains | <0.1% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_928370 |
Additional Formats:
Product Specific Information
- Heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) is recommended for paraffin sections.
- For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
- Mouse anti-Human CD44v6 antibody, clone VFF-7, recognizes an epitope encoded by exon v6 on the variant portion of human CD44.
Target Information
CD44 is a 100 kDa cell surface glycoprotein expressed on human leukocytes, brain white matter, and various epithelial cells. Multiple isoforms exist, including the predominant CD44H isoform. CD44 functions as a receptor for hyaluronic acid (HA) and participates in cell-cell interactions, adhesion, and migration. It plays roles in lymphocyte activation, recirculation, and homing. Upregulation of CD44 has been associated with certain carcinomas and may relate to metastatic potential.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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