Thermo Fisher Scientific CD45RC Monoclonal Antibody (MIL15)

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Flow Cytometry (Flow)

Neat

-

Product Specifications

Species Reactivity

Pig

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

MIL15

Immunogen

Cells isolated from porcine mesenteric lymph node.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

tissue culture supernatant with 5-10% FBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_928466

Product Specific Information

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Pig CD45RC, clone MIL5 recognizes a restricted CD45 epitope encoded by the CD45 C exon and will bind and immunoprecipitate both the approximately 210 kDa RC and approximately 226 kDa RAC isoforms.

Target Information

CD45RC (PTPRC) is a protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. It acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, the second affects the substrate specificity of the first. Upon T-cell activation, CD45RC recruits and dephosphorylates SKAP1 and FYN. Mutations of the gene can result in severe combined immunodeficiency and multiple sclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.