Thermo Fisher Scientific CD44v7 Monoclonal Antibody (VFF-9)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50

-

Immunohistochemistry (Frozen) (IHC (F))

1:50

-

Flow Cytometry (Flow)

1:50

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

VFF-9

Immunogen

Glutathione S Transferase (GST) fusion protein corresponding to the variable domains (v3to v10) of human CD44.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

<0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_928372

Product Specific Information

Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. A suggested positive control for immunohistochemical applications is skin. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Human CD44v7 antibody, clone VFF-9 specifically recognizes an epitope encoded by exon v7 on the variant portion of human CD44.

Target Information

CD44 cell surface antigen is a 100kDa glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.