Thermo Fisher Scientific Nectin 1 Monoclonal Antibody (R1.302)
인간 CD111(Nectin 1)을 인식하는 마우스 단클론 항체로, WB, IHC, ICC, Flow Cytometry 등 다양한 면역분석에 사용 가능. 비결합형 액상 형태이며 Protein A로 정제됨. 4°C 보관, 인간 세포 접착 및 바이러스 수용체 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | 1–4 µg/mL |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | R1.302 |
| Immunogen | Human CD111-transfected NIH/3T3 cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2745553 |
Product Specific Information
This antibody recognizes an extracellular epitope on CD111 (Nectin 1), a 75 kDa type I transmembrane glycoprotein broadly expressed on endothelial cells, epithelial cells, neuronal cells, megakaryocytes, and CD34-positive stem cells.
Target Information
The Nectin 1 gene encodes an adhesion protein involved in the organization of adherens and tight junctions in epithelial and endothelial cells. It is a calcium-independent cell-cell adhesion molecule belonging to the immunoglobulin superfamily, with 3 extracellular immunoglobulin-like loops, a single transmembrane domain, and a cytoplasmic region.
Nectin 1 acts as a receptor for glycoprotein D of herpes simplex viruses 1 and 2, and pseudorabies virus, mediating viral entry into epithelial and neuronal cells. Mutations in this gene are associated with cleft lip and palate/ectodermal dysplasia 1 syndrome and non-syndromic cleft lip with or without cleft palate. Alternative splicing results in multiple transcript variants encoding proteins with distinct C-termini.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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