Thermo Fisher Scientific CD62P (P-Selectin) Monoclonal Antibody (AK4), Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Flow Cytometry (Flow)

2-5 µg/mL

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Chimpanzee, Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

AK4

Immunogen

Human platelets if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:P-Selectin (CD62P),uniProtId:P16109-1,ncbiNodeId:9606,antigenRange:1-830,antigenLength:830,antigenImageFileName:MA5-28596_P-Selectin_CD62P_P16109-1_House_mouse.svg,antigenImageFileNamePDP:MA5-28596_P-Selectin_CD62P_P16109-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

View additional formats

• Unconjugated
• APC
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Size-exclusion chromatography

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2745555

Product Specific Information

This antibody recognizes an extracellular epitope of CD62P (P-selectin), a 140 kD single chain type I transmembrane glycoprotein present in secretory alpha-granules in platelets, in Weibel-Palade bodies in endothelial cells and in megakaryocytes; it is relocated to the plasma membrane upon activation.

Target Information

P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type 1 transmembrane glycoprotein, and is one of the most commonly studied proteins that regulate cell rolling. P-selectin is stored in the Weibel-Palade bodies of endothelial cells, as well as in a-granules of platelets. From there, it can be rapidly brought to the cell surface after exposure to thrombin, histamine, complement 5a, Ca21 ionophores, or adenosine diphosphate. P-selectin protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. P-selectin plays an important role in adhesive processes between leucocytes and endothelial cells, and is a calcium dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens found on neutrophils and monocytes. P-selectin is constitutively expressed in inflammation and contributes to atherogenesis, thrombosis and tissue destruction. Clinically, P-selectin is used to distinguish heparin induced thrombocytopenia with and without thrombosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.