Thermo Fisher Scientific Phospho-SP1 (Thr278) Polyclonal Antibody
SP1 단백질의 Thr278 인산화 형태를 특이적으로 검출하는 rabbit polyclonal antibody. Western blot 및 ICC/IF에 적합하며, 인간·마우스·랫트 반응성. 고순도 정제, 1 mg/mL 농도, PBS/glycerol buffer로 안정적 보관.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000–1:3,000
- View 1 publication
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100–1:500
- View publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human SP1 (Accession P08047), corresponding to amino acid residues around phosphorylated Thr278 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817438 |
Product Specific Information
Antibody detects endogenous levels of SP1 only when phosphorylated at Thr278.
Target Information
SP1 is a transcription factor that can activate or repress transcription in response to physiological and pathological stimuli.
It binds with high affinity to GC-rich motifs and regulates the expression of numerous genes involved in cell growth, apoptosis, differentiation, and immune responses.
SP1 activity is highly regulated by post-translational modifications such as phosphorylation, sumoylation, proteolytic cleavage, glycosylation, and acetylation.
It also binds the PDGFR-alpha G-box promoter and may modulate the cellular response to DNA damage.
SP1 is implicated in chromatin remodeling and plays a role in recruiting SMARCA4/BRG1 on the c-FOS promoter.
It is essential for the regulation of FE65 gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일: PA5-106039_SP1_P08047-1_Rabbit.svg, PA5-106039_SP1_P08047-1_Rabbit_PDP.jpeg)
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