Thermo Fisher Scientific Phospho-SMAD4 (Thr277) Polyclonal Antibody
Phospho-SMAD4 (Thr277) 특이적 폴리클로날 항체로, 인산화된 Smad4 단백질의 검출에 사용됩니다. Western blot, IHC, ICC/IF에 적합하며, 인간, 마우스, 랫트 시료에 반응합니다. PBS/glycerol 기반 용액 형태로 -20°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (IHC) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human SMAD4 (Accession Q13485), corresponding to amino acid residues around phosphorylated Thr277 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817437 |
Product Specific Information
This antibody detects endogenous levels of Smad4 only when phosphorylated at Thr277 (historically referenced as Thr276).
Target Information
Smad4 is encoded by the SMAD4 gene located on chromosome 18 in humans. It is also known as Smad family member 4, Sma- and Mad-related protein 4, or Mothers against decapentaplegic (MAD) homolog 4.
Smad4 contains N-terminal MH1 (MAD homology 1) and C-terminal MH2 (MAD homology 2) domains involved in DNA binding and protein interactions.
Upon activation by TGF-beta or BMP ligands, receptor-regulated Smads (R-Smads: 1, 2, 3, 5, 8) are phosphorylated, form heterotrimers with Smad4 (co-Smad), and translocate to the nucleus to regulate gene expression.
Smad4 acts as a tumor suppressor by modulating genes such as Cyclin D1 (downregulated) and collagen (upregulated), thereby inhibiting cell proliferation.
Dephosphorylation regulates its nuclear export and nucleocytoplasmic dynamics.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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