Thermo Fisher Scientific Endothelin 1 Monoclonal Antibody (TR.ET.48.5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

-

View 27 publications 27 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:250

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:250

-

Immunocytochemistry (ICC/IF)

1:200-1:1,000

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 1 publication 1 publication

Radioimmune Assays (RIA)

1:25,000

-

Product Specifications

Species Reactivity

Human, Mouse, Sheep, Rat

Published species

Dog, Human, Mouse, Pig, Rat, Sheep

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

TR.ET.48.5

Immunogen

Pure endothelin-1 conjugated to KLH.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2096246

Product Specific Information

MA3-005 detects ET-1 from human, sheep, mouse and rat tissues. This antibody shows little cross-reactivity to related peptides and no known cross-reactivity to non-related peptides.

MA3-005 has been successfully used in Western blot, flow cytometry, immunofluorescence, immunohistochemistry, immunocytochemistry, and radioimmune assay procedures. Immunohistochemical staining of ET-1 in human corpus cavernosum tissue with MA3-005 results in staining of endothelial cells. Radioimmune assays using MA3-005 can be used to concentrate ET-1 in solution (e.g. serµm/plasma, milk, urine). This antibody is not recommended for human placenta tissue or HepG2 cells in Western blot applications.

For immunohistochemistry with MA3-005 formalin-fixed, paraffin-embedded tissue sections has been used.

The MA3-005 antigen is purified human endothelin-1. Studies suggest that this antibody binds to an epitope in the region of ET-1 represented by amino acids 8-16.

Target Information

The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.