Thermo Fisher Scientific VCP Monoclonal Antibody (5)
VCP 단백질 검출용 마우스 단클론 항체로 Western blot, IHC, ICC/IF, Flow cytometry 등에 적합합니다. 인간, 마우스, 랫트 시료에서 반응하며, 1.0 mg/mL 농도의 액상 형태로 제공됩니다. Protein G로 정제되어 안정적인 신뢰성 있는 결과를 제공합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 22 publications |
| Immunohistochemistry (IHC) | - | View 11 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:500 | - |
| Immunocytochemistry (ICC/IF) | 1:20–1:200 | View 8 publications |
| Flow Cytometry (Flow) | 1/400 | - |
| Immunoprecipitation (IP) | Assay-dependent | View 4 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse, Yeast |
| Host/Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5 |
| Immunogen | Synthetic peptide corresponding to residues C G(792) G S V Y T E D N D D D L Y G(806) of mouse VCP |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2214638 |
Product Specific Information
MA3-004 detects VCP protein in human, mouse, and rat samples.
This antibody has been validated for Western blot, immunoprecipitation, and immunohistochemical procedures.
By Western blot, it detects an ~97 kDa VCP protein from total lysate of cultured human B cells.
The immunogen used is a synthetic peptide corresponding to residues C G(792) G S V Y T E D N D D D L Y G(806) of mouse VCP (Cat. # PEP-239), which can be used for neutralization and control experiments.
Target Information
The gene encoding VCP belongs to a family of ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and peroxisome assembly.
VCP forms complexes with clathrin and heat-shock protein Hsc70 and plays roles in mitosis-related cellular events such as membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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