Thermo Fisher Scientific VCP Monoclonal Antibody (5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 22 publications 22 publications

Immunohistochemistry (IHC)

-

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:500

-

Immunocytochemistry (ICC/IF)

1:20-1:200

View 8 publications 8 publications

Flow Cytometry (Flow)

1/400

-

Immunoprecipitation (IP)

Assay-dependent

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Yeast

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

5

Immunogen

Synthetic peptide corresponding to residues C G(792) G S V Y T E D N D D D L Y G(806) of mouse VCP. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:VCP,uniProtId:Q01853-1,ncbiNodeId:10090,antigenRange:792-806,antigenLength:806,antigenImageFileName:MA3-004_VCP_Q01853-1_House_mouse.svg,antigenImageFileNamePDP:MA3-004_VCP_Q01853-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2214638

Product Specific Information

MA3-004 detects VCP protein in human, mouse, and rat samples.

MA3-004 has successfully been used in Western blot immunoprecipitation, and immunohistochemical procedures. By Western blot, this antibody detects an ~97 kDa protein representing VCP from total lysate of cultured human B cells.

The MA3-004 immunogen is a synthetic peptide corresponding to residues C G(792) G S V Y T E D N D D D L Y G(806) of mouse VCP. This peptide (Cat. # PEP-239) is available for use in neutralization and control experiments.

Target Information

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.