Thermo Fisher Scientific BAP31 Monoclonal Antibody (CC-4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,500

-

Immunohistochemistry (IHC)

1:200-1:6,400

-

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

1/100

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine, Hamster, Human, Non-human primate

Published species

Human

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

CC-4

Immunogen

Amino acid residues 123-229 of human BAP31. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:BAP31,uniProtId:P51572-1,ncbiNodeId:9606,antigenRange:123-229,antigenLength:246,antigenImageFileName:MA3-003_BAP31_P51572-1_Rat.svg,antigenImageFileNamePDP:MA3-003_BAP31_P51572-1_Rat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_325096

Product Specific Information

MA3-003 detects BAP31 from human, non-human primate, bovine and hamster samples.

MA3-003 has been successfully used in Western blot, immunofluorescent, and immunohistochemical procedures. By Western blot, this antibody detects a 28-kDa protein corresponding to human BAP31.

The MA3-003 antigen is solubilized protein from human neuroendocrine cell line IMR-32, followed by an extract of the small lung human carcinoma cell line SCC-9. MA3-003 binds to a more proximal region of BAP31 (amino acids 123-229). This sequence is conserved in non-human primate, bovine and hamster species.

Target Information

BAP31 (B-Cell Receptor Associated Protein, 31-kDa) is a multiple membrane spanning protein of the endoplasmic reticulum. It has been shown to form both homo- and hetero-oligomers with the closely related BAP29, and is a potential apoptotic regulator as a predicted BCL-2/BCL-XL-associated protein. In the C-terminal domain of BAP31, there are two identical recognition sites for initiator Caspases 1 and 8 of the programmed death cascade. It is hypothesized that BAP31 plays a role in the transport of selected proteins to the Golgi apparatus. BAP31 has also been shown to control expression of CFTR (cystic fibrosis transmembrane conductance regulator). It is believed that interference with the expression or function of BAP31 in epithelial cells may be a way to circumvent the chloride channel defect in cystic fibrosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.