Thermo Fisher Scientific HSP70 Monoclonal Antibody (3A3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 68 publications 68 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

-

Immunocytochemistry (ICC/IF)

1:100

View 5 publications 5 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

-

View 3 publications 3 publications

Immunoprecipitation (IP)

2 µL

View 2 publications 2 publications

Neutralization (Neu)

-

View 1 publication 1 publication

Gel Shift (GS)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Amphibian, Arthropod, Avian, Dog, Fruit fly, Fish, Human, Mollusc, Mouse, Non-human primate, Plant, Pig, Rat, Yeast

Published species

Algae, Amphibian, Arthropod, Crab, Echinoderm, Fish, Fruit fly, Fungi, Human, Insect, Mollusc, Mouse, Non-human primate, Pig, Prawn, Rat, Yeast

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3A3

Immunogen

Human recombinant HSP70 overexpressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325454

Product Specific Information

MA3-006 detects several members of the heat shock protein 70 kDa (HSP70) gene family including HSP70, HSC70, p75, and following heat shock, HSP72 from yeast, Drosophila, fish, porcine, plant, mouse, avian, arthropod (red claw crayfish & black tiger prawn), amphibian, non-human primate, and human samples.

MA3-006 has been successfully used in Western blot, immunocytochemistry, immunofluorescence, gel shift, and immunoprecipitation procedures. By Western blot, this antibody detects proteins from ~70 kDa to ~78 kDa representing different members of the HSP70 family. 2-dimensional gel electrophoresis is required to resolve the heat induced form of these proteins from their constitutively expressed counterparts. Immunocytochemical staining of HSP70 in heat shocked HeLa cells with MA3-006 results in cytoplasmic staining.

The MA3-006 antigen is recombinant human HSP70 over-expressed in E. coli. Epitope mapping with a panel of HSP70 deletion mutants suggests that the epitope recognized is located between amino acids 504-617 of human HSP70, a region that has been shown to be involved in stress-induced nucleolar localization.

Target Information

HSP70 is a Heat shock protein (HSP) which are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs that include HSP70, there are HSP90 and HSP100. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. Further, HSP70 is encoded by an intronless gene and, in conjunction with other heat shock proteins, HSP70 stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and organelles. HSP70 is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The HSP70 gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.