Thermo Fisher Scientific Calpain 2 Monoclonal Antibody (107-82)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 8 publications 8 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:50

-

Immunocytochemistry (ICC/IF)

1:20

-

Flow Cytometry (Flow)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine, Human, Pig, Rabbit, Rat

Published species

Bovine, Fish, Human, Pig, Rabbit, Yeast

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

107-82

Immunogen

Purified bovine skeletal muscle 80 kDa subunit of m-calpain. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Calpain 2,uniProtId:Q27971-1,ncbiNodeId:9913,antigenRange:1-700,antigenLength:700,antigenImageFileName:MA3-942_Calpain_2_Q27971-1_House_mouse.svg,antigenImageFileNamePDP:MA3-942_Calpain_2_Q27971-1_House_mouse_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2290793

Product Specific Information

MA3-942 detects the 80 kDa subunit of m-calpain in human platelets and erythrocytes, bovine platelets, heart and skeletal muscle, rat myoblasts, kidney, liver and spleen, pig cultured cells, and rabbit samples. This antibody does not cross-react with mu-calpain, n-calpain, calmodulin or calpastatin.

MA3-942 has been successfully used in Western blot, IF, immunohistochemistry, and ELISA procedures. By Western blot, this antibody detects an ~80 kDa protein representing m-calpain in bovine platelets. Immunocytochemical staining of m-calpain in LLC-PK1 cells with MA3-942 results in diffuse cytoplasmic staining. This product has not been shown to be effective in immunoprecipitation experiments.

The MA3-942 antigen is purified bovine skeletal muscle 80 kDa subunit of m-calpain. This antibody recognizes an epitope between amino acids 502-699 (domain III/IV) of human calpain.

Target Information

Mu-Calpain, also known as Calpain-I, and m-calpain, also known as Calpain-II, are intracellular, calcium-dependent cysteine proteases. Mu-calpain has a micromolar sensitivity (thus the mu-) as compared to the millimolar calcium sensitivity of m-calpain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.