Thermo Fisher Scientific Calpain 2 Monoclonal Antibody (107-82)
Calpain 2 단백질을 검출하는 Mouse IgG1 단클론 항체로, 인간 및 다양한 종에서 반응. Western blot, IHC, ICC, ELISA 등 다양한 응용 가능. m-calpain 특이적이며 mu-calpain, calmodulin, calpastatin과 교차 반응 없음. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | View 8 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:50 | - |
| Immunocytochemistry (ICC/IF) | 1:20 | - |
| Flow Cytometry (Flow) | Assay-dependent | - |
| ELISA | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Human, Pig, Rabbit, Rat |
| Published Species | Bovine, Fish, Human, Pig, Rabbit, Yeast |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 107-82 |
| Immunogen | Purified bovine skeletal muscle 80 kDa subunit of m-calpain |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2290793 |
Product Specific Information
MA3-942 detects the 80 kDa subunit of m-calpain in human platelets and erythrocytes, bovine platelets, heart and skeletal muscle, rat myoblasts, kidney, liver and spleen, pig cultured cells, and rabbit samples.
This antibody does not cross-react with mu-calpain, n-calpain, calmodulin or calpastatin.
MA3-942 has been successfully used in Western blot, IF, immunohistochemistry, and ELISA procedures.
By Western blot, this antibody detects an ~80 kDa protein representing m-calpain in bovine platelets.
Immunocytochemical staining of m-calpain in LLC-PK1 cells with MA3-942 results in diffuse cytoplasmic staining.
This product has not been shown to be effective in immunoprecipitation experiments.
The MA3-942 antigen is purified bovine skeletal muscle 80 kDa subunit of m-calpain.
This antibody recognizes an epitope between amino acids 502–699 (domain III/IV) of human calpain.
Target Information
Mu-Calpain (Calpain-I) and m-Calpain (Calpain-II) are intracellular, calcium-dependent cysteine proteases.
Mu-calpain has micromolar calcium sensitivity, whereas m-calpain has millimolar calcium sensitivity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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