Thermo Fisher Scientific SPIB Monoclonal Antibody (4G5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

-

Immunocytochemistry (ICC/IF)

1:200-1:1,000

-

Flow Cytometry (Flow)

1:200-1:400

-

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4G5

Immunogen

Purified recombinant fragment of human SPIB (amino acids: 200-252) expressed in E. Coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SPIB,uniProtId:Q01892-1,ncbiNodeId:9606,antigenRange:200-252,antigenLength:262,antigenImageFileName:MA5-17179_SPIB_Q01892-1_House_mouse.svg,antigenImageFileNamePDP:MA5-17179_SPIB_Q01892-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2538650

Product Specific Information

MA5-17179 targets SPIB in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human and Mouse samples.

The MA5-17179 immunogen is purified recombinant fragment of human SPIB (amino acids: 200-252) expressed in E. Coli.

MA5-17179 detects SPIB which has a predicted molecular weight of approximately 28.8kDa.

Target Information

Spi-B is a transcription factor belonging to a subfamily of ETS transcription factors with a conserved ETS domain that mediates specific DNA binding. Spi-B may form homotypic interactions and is known to interact with IRF4, CREBBP, EP300, SPI1/PU.1 related, JUN and TBP. As a sequence specific transcriptional activator it binds to the PU-box, a purine-rich DNA sequence (5-GAGGAA-3) that can act as a lymphoid-specific enhancer. Two isoforms of Spi-B are reported. Spi-B is usually expressed in plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells and promotes their development and block viral replication. Spi-B is not expressed in T-cells or granulocytes but may be present in stem cell populations of the liver and B-cells where it helps in B-cell receptor (BCR) signaling, normal B-cell development and antigenic stimulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.