Thermo Fisher Scientific SPIB Monoclonal Antibody (4G5)
인간 및 생쥐 SPIB 단백질을 검출하는 Mouse IgG1 단일클론 항체입니다. WB, IHC, ICC, Flow Cytometry, ELISA 등 다양한 응용에 적합합니다. 정제된 재조합 SPIB(aa 200-252)을 면역원으로 사용하였으며, 비결합형 액상 형태로 제공됩니다.
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Thermo Fisher Scientific SPIB Monoclonal Antibody (4G5)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4G5 |
| Immunogen | Purified recombinant fragment of human SPIB (amino acids: 200–252) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2538650 |
Product Specific Information
MA5-17179 targets SPIB in indirect ELISA, FACS, ICC, IHC, IF, and WB applications and shows reactivity with human and mouse samples.
The immunogen is a purified recombinant fragment of human SPIB (amino acids 200–252) expressed in E. coli.
MA5-17179 detects SPIB with a predicted molecular weight of approximately 28.8 kDa.
Target Information
Spi-B is a transcription factor belonging to the ETS family with a conserved ETS domain that mediates specific DNA binding.
It interacts with IRF4, CREBBP, EP300, SPI1/PU.1-related proteins, JUN, and TBP. As a sequence-specific transcriptional activator, Spi-B binds to the PU-box (5'-GAGGAA-3'), acting as a lymphoid-specific enhancer.
Spi-B is expressed in plasmacytoid dendritic cells (pDCs) and promotes their development while blocking viral replication.
It is not expressed in T-cells or granulocytes but may be found in liver stem cells and B-cells, aiding B-cell receptor signaling and normal B-cell development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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