Thermo Fisher Scientific PU.1 Monoclonal Antibody (5C7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Flow Cytometry (Flow)

1:200-1:400

-

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5C7

Immunogen

Purified recombinant fragment of human SPI1 (amino acids: 124-271) expressed in E. Coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PU.1,uniProtId:P17947-1,ncbiNodeId:9606,antigenRange:124-270|124-271,antigenLength:270,antigenImageFileName:MA5-17178_PU.1_P17947-1_House_mouse.svg,antigenImageFileNamePDP:MA5-17178_PU.1_P17947-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2538649

Product Specific Information

MA5-17178 targets SPI1 in FACS, indirect ELISA, and WB applications and shows reactivity with Human samples.

The MA5-17178 immunogen is purified recombinant fragment of human SPI1 (amino acids: 124-271) expressed in E. Coli.

MA5-17178 detects SPI1 which has a predicted molecular weight of approximately 42kDa.

Target Information

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.