Thermo Fisher Scientific Osteopontin Monoclonal Antibody (7C5H12)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:200-1:1,000

View 4 publications 4 publications

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human

Published species

Human, Rabbit, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

7C5H12

Immunogen

Purified recombinant fragment of human SPP1 (amino acids: 167-314) expressed in E. Coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Osteopontin,uniProtId:P10451-1,ncbiNodeId:9606,antigenRange:167-314,antigenLength:314,antigenImageFileName:MA5-17180_Osteopontin_P10451-1_House_mouse.svg,antigenImageFileNamePDP:MA5-17180_Osteopontin_P10451-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538651

Product Specific Information

MA5-17180 targets SPP1 in indirect ELISA, ICC, IHC, IF and WB applications and shows reactivity with Human samples.

The MA5-17180 immunogen is purified recombinant fragment of human SPP1 (amino acids: 167-314) expressed in E. Coli.

MA5-17180 detects SPP1 which has a predicted molecular weight of approximately 35.4kDa.

Target Information

Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases close to the integrin-binding Arg-Gly-Asp sequence modulates the function of OPN and its integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage and provide a novel substrate for plasmin and cathepsin D.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.