Thermo Fisher Scientific Osteopontin Monoclonal Antibody (7C5H12)
Human Osteopontin 단백질(SPP1)을 인식하는 Mouse IgG1 단일클론 항체로, WB, IHC, ICC, ELISA 등 다양한 응용에 적합. 1 mg/mL 농도의 액상 형태이며 Protein G로 정제됨. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (IHC) | – | View 9 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 | View 4 publications |
| ELISA | 1:10,000 | View publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Rabbit, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7C5H12 |
| Immunogen | Purified recombinant fragment of human SPP1 (amino acids 167–314) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538651 |
Product Specific Information
MA5-17180 targets SPP1 in indirect ELISA, ICC, IHC, IF, and WB applications and shows reactivity with human samples.
The immunogen is a purified recombinant fragment of human SPP1 (amino acids 167–314) expressed in E. coli.
This antibody detects SPP1 with a predicted molecular weight of approximately 35.4 kDa.
Target Information
Osteopontin is an RGD-containing glycoprotein that interacts with integrins and CD44 as major receptors. It plays key roles in bone mineralization, cell adhesion, migration, chronic inflammation, and transformation.
Proteolytic cleavage by thrombin and matrix metalloproteinases near the integrin-binding Arg-Gly-Asp sequence modulates its function and integrin-binding properties.
Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors, providing a survival advantage and novel substrate for plasmin and cathepsin D.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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