Thermo Fisher Scientific MARVELD2 Recombinant Rabbit Monoclonal Antibody (54H19L38)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-3 µg/mL

View 14 publications 14 publications

Immunohistochemistry (IHC)

-

View 4 publications 4 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1-2 µg/mL

View 7 publications 7 publications

Immunoprecipitation (IP)

5 µg

View 1 publication 1 publication

in situ PLA (PLA)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Dog, Human, Pig

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

54H19L38

Immunogen

A recombinant protein corresponding to amino acids 369-558 of Q8N4S9. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MARVELD2,uniProtId:Q8N4S9-1,ncbiNodeId:9606,antigenRange:369-558,antigenLength:558,antigenImageFileName:700191_MARVELD2_Q8N4S9-1_Rabbit.svg,antigenImageFileNamePDP:700191_MARVELD2_Q8N4S9-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532298

Product Specific Information

This antibody is predicted to react with chimpanzee, mouse, rat, bovine and Xenopus based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Tight junctions form an important barrier of paracellular transport in epithelial cells. Sealing of two adjacent cells at bicellular tight junctions, a point where three adjacent cells are in contact with each other. Tricellulin is the first protein identified that specifically concentrates in tricellular tight junctions. This protein has four membrane spanning domains, similarly to claudins. Tricellulin expression is high in epithelium-derived tissues, such as small intestine, kidney and lung. Functional evidence for the role of tricellulin in tight junction formation comes from siRNA studies, where suppression of its expression leads to compromised epithelial barrier and tight junction formation. Loss of function in tricellulin mutants missing all or most of a conserved region in the cytosolic domain which binds to the cytosolic scaffolding protein ZO-1, indicate that interaction with other known tight junction proteins plays an important role for the function of tricellulin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.