Thermo Fisher Scientific PYGL Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

Antibody detects endogenous levels of total PYGL.

Target Information

This gene encodes a homodimeric protein that catalyzes the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. The protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Its activity is regulated by multiple allosteric effectors and hormonal controls.

Humans have three glycogen phosphorylase genes encoding distinct isozymes expressed primarily in liver, brain, and muscle. The liver isozyme supports systemic glycemic demands, while the brain and muscle forms serve local energy needs. Mutations in the liver glycogen phosphorylase gene cause glycogen storage disease type VI (Hers disease), characterized by moderate hypoglycemia, mild ketosis, growth retardation, and hepatomegaly. Alternative splicing generates multiple transcript variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.