Thermo Fisher Scientific PUMA alpha Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: E(101) Q H L E S P V P S A P G A L A G(117) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PUMA alpha,uniProtId:Q9BXH1-1,ncbiNodeId:9606,antigenRange:101-117,antigenLength:193,antigenImageFileName:PA1-4277_PUMA_alpha_Q9BXH1-1_Rabbit.svg,antigenImageFileNamePDP:PA1-4277_PUMA_alpha_Q9BXH1-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2064549

Product Specific Information

PA1-4277 detects alpha- and beta-PUMA from human samples.

PA1-4277 has been successfully used in Western blot procedures. By Western blot this antibody recognizes both forms of the PUMA protein found at ~20 kDa and ~36 kDa in transfected U20S cells.

PA1-4277 immunizing peptide corresponds to amino acid residues E(101) Q H L E S P V P S A P G A L A G(117) of the human PUMA-a form of the PUMA protein.

Target Information

PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. Antisense inhibition of PUMA expression reduced the apoptotic response to p53, and PUMA is likely to play a role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.