Thermo Fisher Scientific CD56 (NCAM) Monoclonal Antibody (5tukon56), eBioscience

Applications

• Western Blot (WB): Assay-dependent
• Immunohistochemistry (Paraffin) (IHC (P)): 1 µg/mL

Product Specifications

Additional Formats

• Biotin Conjugate
• Request Custom Conjugation

Product Specific Information

This 5tukon56 monoclonal antibody reacts with human CD56, also known as Neural Cell Adhesion Molecule (NCAM).
CD56 is a highly glycosylated transmembrane molecule expressed in both the developing and adult nervous system and plays a role in neurogenesis, migration, neurite extension, and homotypic adhesion of neural cells.
In the hematopoietic system, CD56 is expressed on NK cells and a subset of T cells referred to as NKT cells.

For flow cytometric analysis of human CD56, clones CMSSB or TULY56 are recommended.

Applications Reported:

• Western blotting
• Immunohistochemical staining of formalin-fixed paraffin embedded tissue sections
• Microscopy

Applications Tested:
Tested by IHC of formalin-fixed paraffin embedded tissue sections using low or high pH antigen retrieval.
Recommended use ≤ 1 µg/mL. Antibody should be titrated for optimal performance.

Purity: >90%, determined by SDS-PAGE
Aggregation: <10%, determined by HPLC
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of the immunoglobulin family that serves as an adhesive molecule and is ubiquitously expressed in the nervous system in isoforms ranging from 120–180 kDa.
CD56 is found on T cells and NK cells, and is involved in cell migration, axonal growth, pathfinding, and synaptic plasticity.
Polysialic modification reduces CD56-mediated cell adhesion. Through its extracellular region, CD56 mediates homophilic and heterophilic interactions by binding extracellular matrix components such as laminin and integrins.
CD56 is expressed on most neuroectodermal derived cell lines, tissues, and neoplasms such as retinoblastoma, medulloblastoma, astrocytomas, and neuroblastoma.
Further, CD56 is a widely used neuroendocrine marker with high sensitivity for neuroendocrine tumors and ovarian granulosa cell tumors.
Diseases associated with CD56 dysfunction include rabies and blastic plasmacytoid dendritic cell neoplasms.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.