Thermo Fisher Scientific CD62P (P-Selectin) Monoclonal Antibody (AK-4), eBioscience
인간 CD62P(P-Selectin)을 인식하는 AK-4 단클론 항체로, Flow Cytometry, IHC(F), ICC/IF, ELISA, Neutralization 등 다양한 응용에 적합합니다. 비결합형 액상 형태이며, 높은 순도(>90%)로 제조되어 염증 반응 및 혈소판-백혈구 상호작용 연구에 유용합니다.
- 카탈로그번호
- 14-0628-xx (4개 옵션)
- 판매단위
- pk
카탈로그
4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD62P (P-Selectin) Monoclonal Antibody (AK-4), eBioscience
Thermo Fisher Scientific CD62P (P-Selectin) Monoclonal Antibody (AK-4), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Frozen) (IHC(F)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| Flow Cytometry (Flow) | 1 µg/test |
| ELISA | - |
| Neutralization (Neu) | Assay-dependent |
| Functional Assay | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AK-4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2864912 |
Additional Formats
Product Specific Information
The AK-4 monoclonal antibody reacts with human CD62P, a 140 kDa transmembrane glycoprotein also known as P-Selectin. Upon activation, CD62P is rapidly transported from its cytoplasmic stores in endothelial cells or platelets to the surface, initiating adhesive interactions between endothelial cells and neutrophils or monocytes during inflammation. It also mediates platelet interactions with monocytes and neutrophils.
Applications Reported:
- Flow cytometric analysis
- Immunohistochemical staining of frozen tissue sections
- Blocking adhesion of platelets in vitro
Applications Tested:
Tested by flow cytometric analysis of resting and thrombin-activated human platelets. Recommended usage ≤1 µg/test (100 µL final volume). Cell count should be empirically determined (10⁵–10⁸ cells/test). Optimal performance requires titration.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type I transmembrane glycoprotein involved in cell rolling and adhesion. Stored in Weibel-Palade bodies of endothelial cells and α-granules of platelets, it translocates to the cell surface upon activation by thrombin, histamine, complement 5a, Ca²⁺ ionophores, or ADP. P-selectin mediates interactions between activated endothelial cells or platelets and leukocytes, contributing to inflammatory processes, atherogenesis, thrombosis, and tissue destruction. Clinically, it assists in distinguishing heparin-induced thrombocytopenia with and without thrombosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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