Thermo Fisher Scientific CD62P (P-Selectin) Monoclonal Antibody (AK-4), eBioscience

Thermo Fisher Scientific CD62P (P-Selectin) Monoclonal Antibody (AK-4), eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Formats

• FITC Conjugate
• PE Conjugate
• Request Custom Conjugation

Product Specific Information

The AK-4 monoclonal antibody reacts with human CD62P, a 140 kDa transmembrane glycoprotein also known as P-Selectin. Upon activation, CD62P is rapidly transported from its cytoplasmic stores in endothelial cells or platelets to the surface, initiating adhesive interactions between endothelial cells and neutrophils or monocytes during inflammation. It also mediates platelet interactions with monocytes and neutrophils.

Applications Reported:

• Flow cytometric analysis
• Immunohistochemical staining of frozen tissue sections
• Blocking adhesion of platelets in vitro

Applications Tested:
Tested by flow cytometric analysis of resting and thrombin-activated human platelets. Recommended usage ≤1 µg/test (100 µL final volume). Cell count should be empirically determined (10⁵–10⁸ cells/test). Optimal performance requires titration.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type I transmembrane glycoprotein involved in cell rolling and adhesion. Stored in Weibel-Palade bodies of endothelial cells and α-granules of platelets, it translocates to the cell surface upon activation by thrombin, histamine, complement 5a, Ca²⁺ ionophores, or ADP. P-selectin mediates interactions between activated endothelial cells or platelets and leukocytes, contributing to inflammatory processes, atherogenesis, thrombosis, and tissue destruction. Clinically, it assists in distinguishing heparin-induced thrombocytopenia with and without thrombosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.