Thermo Fisher Scientific CD62E (E-selectin) Monoclonal Antibody (P2H3), eBioscience
CD62E(E-selectin) 단클론 항체(P2H3)는 인간 및 마우스 시료에서 염증 반응 시 내피세포 표면의 E-selectin을 검출합니다. Flow cytometry, IHC, IP 등 다양한 응용에 적합하며, 0.5 mg/mL 농도의 액상 형태로 제공됩니다. TNFα 자극 HUVEC 분석에 검증되었습니다.
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- 14-0627-xx (4개 옵션)
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카탈로그
4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD62E (E-selectin) Monoclonal Antibody (P2H3), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | - |
| Immunohistochemistry (IHC) | Assay-Dependent | - |
| Flow Cytometry (Flow) | 1 µg/test | 3 publications |
| ELISA | - | 1 publication |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Neutralization (Neu) | - | 1 publication |
| Functional Assay | Assay-Dependent | 1 publication |
| Inhibition Assays (IA) | - | 2 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | P2H3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2864910 |
Available Formats
Product Specific Information
The P2H3 monoclonal antibody reacts with human CD62E, a 97–115 kDa member of the selectin family. CD62E (E-selectin or ELAM-1) is an adhesion molecule expressed by endothelial cells upon stimulation with cytokines such as TNFα and IL-1β. Its expression mediates leukocyte rolling and the initial interaction of neutrophils with endothelium during inflammation.
This antibody inhibits cellular adhesion to cytokine-activated endothelial cells.
Applications Reported:
Flow cytometry, immunoprecipitation, and immunohistochemistry.
Applications Tested:
Validated by flow cytometric analysis of TNFα-activated Human Umbilical Vein Endothelial Cells (HUVEC). Recommended ≤1 µg per test (100 µL final volume). Optimal titration should be determined empirically.
Purity: >90% by SDS-PAGE
Aggregation: <10% by HPLC
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Leukocytes adhere to the endothelium during extravasation in postcapillary venules and at inflammatory sites. This process involves adhesion receptors from the selectin and immunoglobulin superfamilies. E-selectin (CD62E) is an endothelial-specific selectin induced by proinflammatory cytokines such as IL-1, TNFα, and bacterial components (e.g., LPS) via NFκB signaling. It plays a key role in leukocyte adhesion during inflammation and is associated with vascular endothelial activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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