Thermo Fisher Scientific IRF3 Recombinant Rabbit Monoclonal Antibody (SD2062)
IRF3 단백질을 인식하는 재조합 토끼 단일클론 항체. Western blot, IHC, ICC, Flow cytometry 등 다양한 분석에 사용 가능. 높은 특이성과 민감도, 로트 간 일관성, 동물 유래 성분 무함유 포뮬레이션 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1000–1:5000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:2000 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | - |
| Flow Cytometry (Flow) | 1:1000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SD2062 |
| Immunogen | Synthetic peptide within Human IRF3 aa 71–120 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809629 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
IRF3 (Interferon Regulatory Factor 3) is a key transcriptional regulator of type I interferon (IFN)-dependent immune responses. It plays a critical role in the innate immune response against DNA and RNA viruses.
IRF3 regulates transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to interferon-stimulated response elements (ISRE) in their promoters. It acts as a potent activator of IFN-beta gene and functions in both early and late phases of IFNA/B gene induction.
In uninfected cells, IRF3 remains inactive in the cytoplasm. Upon viral infection or toll-like receptor signaling, it is phosphorylated by IKBKE and TBK1 kinases, leading to dimerization, nuclear localization, and association with CREB binding protein to form dsRNA-activated factor 1 (DRAF1). This complex activates transcription of type I IFN and ISG genes.
Mutations in IRF3 can result in acute infection-induced encephalopathy (herpes-specific type 7).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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