Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), NovaFluor Red 710, eBioscience

Applications

• Flow Cytometry (Flow): Tested at 5 µL (0.2 µg)/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661 / 737 / 805
• Brilliant Violet 480
• eFluor 450 / 506
• FITC
• Functional Grade
• NovaFluor Blue 610-70S / 660-120S
• NovaFluor Red 725
• NovaFluor Yellow 660 / 730
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation available

Product Specific Information

The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), a low-affinity receptor for IgG with an apparent molecular weight of 50–80 kDa. CD16 is encoded by two related genes:

• CD16A (FcγRIIIA): Expressed on macrophages and NK cells (transmembrane form)
• CD16B (FcγRIIIB): Expressed on neutrophils (GPI-anchored form)

Two polymorphisms, NA-1 and NA-2, exist for CD16B; NA-2 homozygotes show reduced phagocytic capacity. CD16 binds immune complexes of IgG, preferentially IgG1 and IgG3, and mediates ADCC, phagocytosis, degranulation, and proliferation upon activation.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported

• Suitable for flow cytometric analysis of human peripheral blood cells.

Applications Tested

• Pre-diluted and validated at 5 µL (0.2 µg) per test (100 µL final volume).
• Cell number range: 10⁵–10⁸ cells/test.

Compatibility Information

• NovaFluor dyes are not compatible with DNA-intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

CellBlox Plus Blocking Buffer

• Updated conjugate ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01).
• Required for optimal staining with NovaFluor conjugates.
• Add 5 µL per sample to antibody cocktails before cell addition.
• For single-color controls: 5 µL per 100 µL of sample (10³–10⁸ cells).

NovaFluor Technology

NovaFluor conjugates utilize Phiton™ technology, based on novel nucleic acid dye structures that enable engineered fluorescent signatures with reduced spillover and spread.

• Excitation: 639 nm
• Emission: 710 nm
• Laser: 633–640 nm (Red)

Learn more about NovaFluor dyes

Target Information

CD16 (FcγRIII) encodes a receptor for the Fc portion of IgG, involved in:

• Clearance of immune complexes
• Antibody-dependent cytotoxicity
• Enhancement of viral infection responses

The FCGR3A gene encodes the NK cell–associated transmembrane receptor, while FCGR3B encodes the neutrophil-specific GPI-linked receptor.
Mutations are linked to Immunodeficiency 20, Herpes Zoster, and Systemic Lupus Erythematosus susceptibility.
Alternative splicing generates multiple isoforms.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

제품 이미지