Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), NovaFluor Red 710, eBioscience

Applications

• Flow Cytometry (Flow): Tested at 5 µL (0.2 µg)/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661 / 737 / 805
• Brilliant Violet 480
• eFluor 450 / 506
• FITC
• Functional Grade
• NovaFluor Blue 610-70S / 660-120S
• NovaFluor Red 725
• NovaFluor Yellow 660 / 730
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation available

Product Specific Information

The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), a low-affinity receptor for IgG with an apparent molecular weight of 50–80 kDa. CD16 is encoded by two related genes:

• CD16A (FcγRIIIA): Expressed on macrophages and NK cells (transmembrane form)
• CD16B (FcγRIIIB): Expressed on neutrophils (GPI-anchored form)

Two polymorphisms, NA-1 and NA-2, exist for CD16B; NA-2 homozygotes show reduced phagocytic capacity. CD16 binds immune complexes of IgG, preferentially IgG1 and IgG3, and mediates ADCC, phagocytosis, degranulation, and proliferation upon activation.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported

• Suitable for flow cytometric analysis of human peripheral blood cells.

Applications Tested

• Pre-diluted and validated at 5 µL (0.2 µg) per test (100 µL final volume).
• Cell number range: 10⁵–10⁸ cells/test.

Compatibility Information

• NovaFluor dyes are not compatible with DNA-intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

CellBlox Plus Blocking Buffer

• Updated conjugate ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01).
• Required for optimal staining with NovaFluor conjugates.
• Add 5 µL per sample to antibody cocktails before cell addition.
• For single-color controls: 5 µL per 100 µL of sample (10³–10⁸ cells).

NovaFluor Technology

NovaFluor conjugates utilize Phiton™ technology, based on novel nucleic acid dye structures that enable engineered fluorescent signatures with reduced spillover and spread.

• Excitation: 639 nm
• Emission: 710 nm
• Laser: 633–640 nm (Red)

Learn more about NovaFluor dyes

Target Information

CD16 (FcγRIII) encodes a receptor for the Fc portion of IgG, involved in:

• Clearance of immune complexes
• Antibody-dependent cytotoxicity
• Enhancement of viral infection responses

The FCGR3A gene encodes the NK cell–associated transmembrane receptor, while FCGR3B encodes the neutrophil-specific GPI-linked receptor.
Mutations are linked to Immunodeficiency 20, Herpes Zoster, and Systemic Lupus Erythematosus susceptibility.
Alternative splicing generates multiple isoforms.

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For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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