Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), NovaFluor Red 725, eBioscience

Applications

• Tested Application: Flow Cytometry (Flow)
• Recommended Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661 / 737 / 805
• Brilliant Violet 480
• eFluor 450 / 506
• FITC
• Functional Grade
• NovaFluor Blue 610-70S / 660-120S
• NovaFluor Red 710
• NovaFluor Yellow 660 / 730
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Custom conjugation available

Product Specific Information

The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), a low-affinity receptor for IgG (50–80 kDa).
CD16 includes two genes:

• CD16A (FcγRIIIA): Found in macrophages and NK cells, transmembrane anchored
• CD16B (FcγRIIIB): Found in neutrophils, GPI-anchored

Two known polymorphisms, NA-1 and NA-2, affect phagocytic capacity. CD16 binds IgG1 and IgG3 preferentially, mediating ADCC, phagocytosis, degranulation, and proliferation upon immune complex binding.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for human peripheral blood cells; use 5 µL (0.6 µg) per 100 µL test volume (10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining.
  • Add 5 µL per sample before combining antibodies with cells.
  • For single-color controls, use 5 µL per 100 µL of cell sample.

Technology:
NovaFluor conjugates utilize Phiton™ technology, engineered for minimal spillover and optimal spectral separation.

Excitation: 636 nm
Emission: 727 nm
Laser: 633–640 nm (Red)

Target Information

CD16 (FCGR3A) encodes the Fc receptor for IgG, mediating immune complex clearance, ADCC, and viral infection enhancement.

• FCGR3A: Expressed on NK cells, transmembrane anchored
• FCGR3B: Expressed on neutrophils, PI-linked

Mutations are associated with Immunodeficiency 20, Herpes Zoster, and Systemic Lupus Erythematosus (SLE).
Alternative splicing produces multiple isoforms.

For Research Use Only. Not for diagnostic use or resale without authorization.

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