Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), NovaFluor Blue 660-120S, eBioscience

Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), NovaFluor Blue 660-120S, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

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Product Specifications

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Additional Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661 / 737 / 805
• Brilliant Violet 480
• eFluor 450 / 506
• FITC
• Functional Grade
• NovaFluor Blue 610-70S / Red 710 / Red 725 / Yellow 660 / Yellow 730
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation available

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Product Specific Information

The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), a low-affinity receptor for IgG (50–80 kDa). CD16 includes two genes:

• CD16A (FcγRIIIA): Found in macrophages and NK cells (polypeptide-anchored form)
• CD16B (FcγRIIIB): Found in neutrophils (GPI-anchored form)

Polymorphisms NA-1 and NA-2 affect phagocytic capacity, with NA-2 showing reduced activity. CD16 binds IgG immune complexes, preferentially IgG1 and IgG3, initiating signal cascades for ADCC, phagocytosis, degranulation, and proliferation.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on human peripheral blood cells
Recommended usage: 5 µL (0.6 µg) per test in 100 µL total volume (10⁵–10⁸ cells/test).

Important Note:
NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

CellBlox Plus Blocking Buffer:
Updated buffer (Cat. No. C001T06F01) required for optimal staining with NovaFluor conjugates.

• Add 5 µL per sample before mixing with cells.
• For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates use Phiton™ technology with engineered fluorescent signatures designed to minimize spillover and spread.

Excitation: 509 nm
Emission: 665 nm
Laser: 488 nm (Blue)

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Target Information

CD16 encodes a receptor for the Fc portion of IgG, mediating immune complex clearance and functions such as cytotoxicity and viral infection enhancement.

• FCGR3A: Expressed on NK cells (membrane-anchored)
• FCGR3B: Expressed on neutrophils (PI-anchored)

Mutations may cause Immunodeficiency 20, Herpes Zoster susceptibility, and Systemic Lupus Erythematosus. Alternative splicing generates multiple isoforms.

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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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