Thermo Fisher Scientific IL-1 beta Polyclonal Antibody, HRP
IL-1 beta 단백질 검출용 HRP 결합 폴리클로날 항체로, Western blot, IHC, ELISA 등 다양한 면역분석에 적합합니다. 인간 및 영장류 시료 반응성이 높으며, 고순도 DEAE 정제 IgG 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific IL-1 beta Polyclonal Antibody, HRP
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,500 |
| ELISA | 1:10,000–1:50,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Non-human primate |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1.1 mg/mL |
| Purification | Size-exclusion (Gel filtration) |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Immunogen
This antibody was prepared by repeated immunizations with recombinant human IL-1 beta produced in E. coli.
The recombinant 153 aa IL-1 beta has a molecular weight of 17 kDa, with the N-terminal amino acid at position alanine 117.
This cleavage site is generated by the IL-1 beta converting enzyme (ICE, caspase-1).
Product Specific Information
- Store vial at 4°C prior to restoration. For extended storage, aliquot and freeze at -20°C or below. Avoid freeze/thaw cycles.
- Centrifuge if solution is not clear after standing. Stable for several weeks at 4°C as undiluted liquid.
- Heated to 56°C for 30 minutes; purified by DEAE fractionation.
- Primarily directed against mature 17 kDa human IL-1 beta; also detects primate IL-1 beta.
- Does not recognize human IL-1 alpha.
- In ELISA assays, detects mainly the mature 17 kDa form and partially the native 31 kDa precursor.
- In immunoblots, detects both 17 kDa (mature) and ~35 kDa (denatured precursor) bands.
Target Information
Interleukin-1 beta (IL-1β) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells.
It is synthesized as a 31 kDa inactive pro-form and processed into an active 17 kDa protein via inflammasome activation and caspase-1 cleavage.
IL-1β lacks a signal peptide and is secreted through a non-classical pathway.
It signals through IL-1RI and IL-1RII, shared with IL-1α, and its activity can be inhibited by IL-1 Receptor Antagonist (IL-1RA).
IL-1β plays a key role in innate host defense and inflammation; elevated levels are linked to chronic inflammatory diseases.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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